Please follow https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?INPUT_SEQUENCE=NG_009629.1&PRIMER5_START=12918&PRIMER5_END=13018&PRIMER3_START=13188&PRIMER3_END=13288&ORGANISM=9606&PRIMER_SPECIFICITY_DATABASE=PRIMERDB/genome_selected_species.