Please follow https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?INPUT_SEQUENCE=NM_000250&ORGANISM=Homo%20sapiens&PRIMER_SPECIFICITY_DATABASE=refseq_mrna&SPAN_INTRON=on.

Please follow https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?INPUT_SEQUENCE=NM_000250&ORGANISM=Homo%20sapiens&PRIMER_SPECIFICITY_DATABASE=refseq_mrna&SPAN_INTRON=on.